A standard 90 degree V-8 has a burble due to irregular firing intervals. But with a cylinder down, it sounds very different.
A well designed twin pipe system will have a balance pipe between them. To even out the extraction effect. But even without, all you've done is half the choices.
A decent rev counter will show which cylinder is poor by unplugging each plug in turn, and noting the engine speed. But you should be able to hear this anyway.